Summary
The Activator I and II solutions provide the substrate for the ELISPOT assay based on silver staining and should be used in conjunction with GABA conjugate (CT351). Prior to use and as final step in the ELISPOT procedure, the solutions should be mixed (1:1) and added to the wells of the ELISPOT plate. The substrate will produce a microscopally black and visually yellow/brown spot. Upon ordering, you will receive one vial containing 9.5 ml Activator I and one vial containing 9.5 ml Activator II for 480 ELISPOT determinations.