Description:
Mouseinterferon-gammaELISPOT:
IFN-gammaspecificspotsproducedby2.5x10E4mouse(BALB/c)splenocytes.
Stimulation:PMA/ionomycin.
Platecharacteristics:
PVDF
Contents:
- Coatingantibodies
- Biotinylateddetectorantibodies
- Streptavidin-HorserADIshPeroxidase
- AECcoloringsystem
- Blockingstocksolution
- DilutionbufferR
- Tween-20
- PVDFELISPOTplateswithlidsandcoverslips
- Detailedmanual
| Referenceslist:
| - VaccinationofmicewithrecombinantbaculovirusexpressingspikeornucleocapsidproteinofSARS-likecoronavirusgenerateshumoralandcellularimmuneresponses.Bai,Bingke,etal.Mol.Immunol.2008Feb;45:868-75
- Genetherapyfortolerance:high-levelexpressionofdonormajorhistocompatibilitycomplexintheliverovercomesnaiveandmemoryalloresponsestoskingrafts.Cunningham,EithneC,etal.Transplantation2013Jan15;95:70-7
- Thepreparationofhumanpapillomavirustype58vaccineandexploringitsBIOLOGicalactivityandimmunogenicityinvitro.Deng,Dongrui,etal.Int.J.Gynecol.Cancer2011Aug;21:988-95
- IdentificationofcytotoxicTlymphocyteepitopesindenguevirusSEROtype1.Duan,Zhiliang,etal.J.Med.Virol.2015Jul;87:1077-89
- DevelopmentofamoreefficienthepatitisBvirusvaccinebytargetinghepatitisBviruspreStodendriticcells.Jing,Mingzhen,etal.Vaccine2016Jan20;34:516-22
- SyntheticpeptidescontainingB-andT-cellepitopeofdenguevirus-2EdomainIIIprovokedB-andT-cellresponses.Li,Shanfeng,etal.Vaccine2011May9;29:3695-702
- IncreasedantigenpresentationbutimpairedTcellsprimingafterupregulationofinterferon-betainducedbylipopolysaccharidesismediatedbyupregulationofB7H1andGITRL.Wang,Fang,etal.PLoSONE2014;9:e105636
- ChimaericproteinimprovedimmunogenicitycomparedwithfusionproteinofAg85BandESAT-6antigensofMycobacteriumtuberculosis.Xu,Y,etal.Scand.J.Immunol.2006Nov;64:476-81
- KeyroleoftheGITR/GITRLigandpathwayinthedevelopmentofmurineautoimmunediabetes:apotentialtherapeutictarget.You,Sylvaine,etal.PLoSONE2009;4:e7848
- HepatitisBviruscoreantigenepitopespresentedbyHLA-A2single-chaintrimersinducefunctionalepitope-specificCD8+T-cellresponsesinHLA-A2.1/Kbtransgenicmice.Zhang,Yuxia,etal.Immunology2007May;121:105-12
u-cytech Biosciences公司成立于1999与乌特列支大学合作的生物科技公司和位于乌得勒支的荷兰(欧洲)。该家族企业以开放的政策和众多忠实的顾客从事细胞因子生物学的基础研究和应用研究,致力于在ELISA和T、B细胞的ELISPOT检测而设计的个别蛋白质分泌细胞的体外检测。此外,我们开发的“房子”一个广泛的高度特异的单克隆抗体和多克隆抗体的蛋白质包括不同的物种形成了我们高度敏感的检测的基础上(ELISA、ELISPOT、FluoroSpot)。
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