The monoclonal antibody to mouse IL-4 (clone 11B11) has shown to be suitable for intracellular staining (Assenmacher M. et al. 1998), ELISA (Carson R.T. et al. 1999) and Western Blot. The 11B11 antibody can also successfully be used in immunohistochemistry and neutralization assays (Chaudhri G. et al. 2004 and Hammond K.J. et al. 1998).
References list
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
Assenmacher, M, et al.J. Immunol. 1998 Sep 15;161: 2825-32
Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay.
Carson, R T, et al.J. Immunol. Methods 1999 Jul 30;227: 41-52
Polarized type 1 cytokine response and cell-mediated immunity determine genetic resistance to mousepox.
Chaudhri, Geeta, et al.Proc. Natl. Acad. Sci. U.S.A. 2004 Jun 15;101: 9057-62
Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
Cherwinski, H M, et al.J. Exp. Med. 1987 Nov 01;166: 1229-44
An immunoadhesin incorporating the molecule OX-2 is a potent immunosuppressant that prolongs allo- and xenograft survival.
Gorczynski, R M, et al.J. Immunol. 1999 Aug 01;163: 1654-60
Administration of anti-IL-4 monoclonal antibody 11B11 increases the resistance of mice to Listeria monocytogenes infection.
Haak-Frendscho, M, et al.J. Immunol. 1992 Jun 15;148: 3978-85
alpha/beta-T cell receptor (TCR)+CD4-CD8- (NKT) thymocytes prevent insulin-dependent diabetes mellitus in nonobese diabetic (NOD)/Lt mice by the influence of interleukin (IL)-4 and/or IL-10.
Hammond, K J, et al.J. Exp. Med. 1998 Apr 06;187: 1047-56
Involvement of Th2 cytokines in the mouse model of flutamide-induced acute liver injury.
Higuchi, Satonori, et al.J Appl Toxicol 2012 Oct;32: 815-22
IL-4 mediates dicloxacillin-induced liver injury in mice.
Higuchi, Satonori, et al.Toxicol. Lett. 2011 Feb 05;200: 139-45
Th2 cytokine-mediated methimazole-induced acute liver injury in mice.
Kobayashi, Masanori, et al.J Appl Toxicol 2012 Oct;32: 823-33
Cholera toxin B subunit binding to an antigen-presenting cell directly co-stimulates cytokine production from a T cell clone.
Li, T K, et al.Int. Immunol. 1996 Dec;8: 1849-56
Isolation of monoclonal antibodies specific for IL-4, IL-5, IL-6, and a new Th2-specific cytokine (IL-10), cytokine synthesis inhibitory factor, by using a solid phase radioimmunoadsorbent assay.
Mosmann, T R, et al.J. Immunol. 1990 Nov 01;145: 2938-45
Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1.
Ohara, J, et al.Nature 1985 May 23-29;315: 333-6
B-cell stimulatory factor-1/interleukin 4.
Paul, W E, et al.Annu. Rev. Immunol. 1987;5: 429-59