The rat anti-mouse IL-5 monoclonal antibody (clone TRFK5) was first described in 1988. The TRFK5 antibody recognizes and neutralizes both mouse and human IL-5 (Schumacher J.H. et al. 1988). The antibody has been descriped as useful in a sandwich ELISA (Schumacher J.H. et al. 1988) and the ELISPOT assay (Taguchi T. et al. 1990). The TRFK5 antibody has also been reported for use in intracellular staining (Belkaid Y. et al. 1998), immunohistochemical staining (Fan W.Y. et al. 2001) and Western Blot (Zhang J. et al. 1999).
References list
- Development of a natural model of cutaneous leishmaniasis: powerful effects of vector saliva and saliva preexposure on the long-term outcome of Leishmania major infection in the mouse ear dermis.
- Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice.
- The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
- Detection of individual mouse splenic T cells producing IFN-gamma and IL-5 using the enzyme-linked immunospot (ELISPOT) assay.
- Mapping and characterization of the epitope(s) of Sch 55700, a humanized mAb, that inhibits human IL-5.
u-cytech Biosciences公司成立于1999与乌特列支大学合作的生物科技公司和位于乌得勒支的荷兰（欧洲）。该家族企业以开放的政策和众多忠实的顾客从事细胞因子生物学的基础研究和应用研究，致力于在ELISA和T、B细胞的ELISPOT检测而设计的个别蛋白质分泌细胞的体外检测。此外，我们开发的“房子”一个广泛的高度特异的单克隆抗体和多克隆抗体的蛋白质包括不同的物种形成了我们高度敏感的检测的基础上（ELISA、ELISPOT、FluoroSpot）。
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